INTRODUCTION
Department of Pathology and Laboratory Medicine at Deen Dayal Upadhyay Hospital (DDUH) is well equipped to cater to the needs of outdoor patients and indoor patients under one roof. Department of Pathology and Laboratory Medicine is constituted of various sections:
· Biochemistry
· Hematology
· Cytology
· Histopathology
· Clinical Pathology
· Thalassemia Screening Centre
· Microbiology including ICTC with Hospital infection control programme.
The Faculty includes senior consultant, specialists, senior residents, medical officers and junior residents
FACULTY
Pathology
S.No. |
Name |
Present Post |
Mobile No. |
Landline (Ext.) |
1 |
Dr.Neelam Sood |
Consultant and HOD Dept. of Pathology and Lab Medicine |
9718990230 |
531 |
2. |
Dr.Rashmi Yadav |
Specialist –Gr-I |
9718990231 |
538 |
3. |
Dr.Jaya Malhotra |
Specialist –Gr-I |
9718990232 |
539 |
4. |
Dr.Garima Goyal |
Jr. Specialist |
9718990237 |
532 |
5. |
Dr.Nidhi Chauhan |
Specialist –Gr-III |
8744061144 |
532 |
1 |
Dr.Ram Chandra |
M.O. and Incharge Emergency Lab |
9718932004 |
243 |
Biochemistry
S.No. |
Name |
Present Post |
Mobile No. |
Landline (Ext.) |
1. |
Dr.Bhawesh Mishra |
Specialist –Gr-III |
9953930848 |
243 |
Microbiology
S.No. |
Name |
Present Post |
Mobile No. |
Landline (Ext.) |
1 |
Dr. Shalini Kakar |
Specialist –Gr-I and Incharge Microbiology |
9718990233 |
512 |
2. |
Dr.Anupama Mittal |
Specialist –Gr-I |
9718990234 |
512 |
3. |
Dr.Poonam Gupta |
Jr. Specialist |
9718990239 |
512 |
4. |
Dr.Vijay Maurya |
Specialist |
|
|
DDU HOSPITAL, HARI NAGAR, NEW DELHI
LIST OF INVESTIGATION IN THE DEPARTMENT OF PATHOLOGY & LAB MEDICINE
COLLECTION TIME: Routine OPD- Monday to Friday 9.0-11.15AM, Saturday 9.0-11.0AM
Name, Age, Sex, CR no., Ward & Bed No., Date, Cl. History & Diagnosis, Doctor’s Name—Mandatory On Requisition Slip
S.no. |
NAME OF SECTION |
INSTRUCTIONS | ||
1. |
HAEMATOLOGY ( Room no. P-2) |
Samples for Haematology to be sent in EDTA | ||
|
Haemoglobin (Hb) |
(purple top) vial in Room no. P-2 | ||
|
Hb, TLC, DLC |
| ||
|
Hb, TLC,DLC, TPC |
| ||
|
Hb with PCV |
| ||
|
Hb, BG (blood group) |
| ||
|
Haemogram with P/S |
| ||
|
Haemogram with P/S |
| ||
|
Haemogram with or without ESR |
| ||
|
Absolute Eosinophil Count (AEC) |
| ||
|
Total Platelet Count (TPC) |
| ||
|
FDP |
| ||
|
Hb, TLC, DLC with MP/RMAT/Microfilaria |
| ||
|
Haemogram with Reticulocyte Count(RC) |
| ||
|
NESTROFT (Thallasemia Screening) |
| ||
2. |
SPECIAL HAEMATOLOGY (Room no. P-2) |
| ||
|
Bone Marrow Examination- for malignancy, anaemia, LD bodies, Iron stores etc. |
Bone marrow smears to be sent with sample for P/S | ||
|
Qualitative Test For G6PD Deficiency |
On Friday (Prior Appointment) | ||
|
Sickling Test for Sickle Cell Anaemia |
With Prior Appointment | ||
|
LE Cell Test- On Friday |
With Prior Appointment | ||
|
Prothrombin Time (PT), Partial Thromboplastin Time (PTT) |
In Citrate (blue top) vial | ||
|
Coomb’s Test Direct/ Indirect |
On WEDNESDAY (Prior Appointment) | ||
|
HPLC ( Thallasemia Screening) |
Room No. P-9 (Prior Appointment) | ||
3. |
CLINICAL PATHOLOGY (Room no. P-7) |
| ||
|
Urine examination (Routine & Microscopic) |
Container for urine should be clean & sample to be given in room n. P-7 | ||
|
24hr Urine Protein |
| ||
|
Urine Bile Salt, Bile Pigment |
| ||
|
Urine For Bence Jones Protein (Prior Appointment) |
| ||
|
Stool for Ova & Cyst |
| ||
|
Stool for Occult Blood |
| ||
|
Semen Analysis(Total Count, Morphology,Motility |
| ||
|
Urine Test for Pregnancy |
| ||
4. |
BIOCHEMISTRY (Room no. P-4&P-5) |
| ||
|
Blood Sugar-F,PP,Random |
Sample for sugar in fluoride(grey top) vial | ||
|
Glucose Tolerance Test (GTT),GCT |
| ||
|
Blood Urea, Serum Creatinine |
Sample for rest of Biochemistry should be sent in | ||
|
RBS,LFT,KFT, Lipid Profile, S. Electrolyte |
Plain (yellow top) vial in room no. P-5 | ||
|
Serum Cholesterol |
| ||
|
Serum Bilirubin |
| ||
|
Serum Alkaline Phosphate (ALP) |
| ||
|
Serum Uric Acid |
| ||
|
Bilirubin, S.G.O.T., S.G.P.T. |
| ||
|
LFT(Bilirubin, SGOT,SGPT,ALP &Proteins) |
| ||
|
KFT (Urea Creatinine,Uric acid, Calcium,phosphorus Electrolytes) |
| ||
|
Lipid Profile- (cholesterol, HDL, LDL, VLDL,TG) |
| ||
|
Serum Amylase, Lipase |
| ||
|
Serum Electrolytes- Na,K, Cl, Ca+ |
| ||
|
CPK-MB, CPK,LDH |
| ||
|
Serum Proteins A:G Ratio |
| ||
|
Protein Electrophoresis |
On Wednesday (Prior appointment) | ||
|
Calcium, Phosphorus, ALP |
| ||
|
CRP,RA, ASO |
| ||
|
HbA1c |
On Wednesday (Prior appointment) Sample in EDTA | ||
|
Urine for microalbumin |
On Wednesday (Prior appointment) | ||
5. |
EMERGENCY LAB. :- (Room no. P-6, Emergency block) |
Sample and report receiving to be done through register | ||
|
Haemoglobin (Hb) |
Sample for haematology to be sent in EDTA | ||
|
Hb , BG(blood group) |
(purple top) vial | ||
|
Hb, TLC, DLC |
| ||
|
Hb, TLC, DLC, TPC |
| ||
|
Total platelet count (TPC) |
| ||
|
P/S for Malarial parasite (MP)/ Microfilaria |
| ||
|
Haemogram with P/S (9AM TO 7PM) |
| ||
|
CRP |
In plain vial | ||
|
FDP |
In plain vial | ||
|
CPK, CPK-MB |
Sample for sugar in fluoride (grey top) vial | ||
|
S. lipase, S. Amylase |
Sample for rest of Biochemistry should be sent in | ||
|
Blood Sugar, Urea, Bilirubin, Creatinine |
Plain (yellow top) vial | ||
|
S. Electrolytes |
| ||
|
CSF |
CSF- In red and purple top vials(Both) | ||
|
Urine for ketones |
| ||
6. |
CYTOLOGY :-(Room no. P11\P14 |
| ||
|
Fine Needle Aspiration Cytology (FNAC) |
(Prior appointment) | ||
|
USG Guided/CT Guided FNAC |
(Prior appointment) | ||
|
Nipple Discharge Smears |
| ||
|
Fluid Cytology (Pleural, Synovial etc.) |
| ||
|
Buccal Smear |
| ||
|
Urine/Sputum for malignant cells |
| ||
|
PAP Smear in Cancer Clinic |
| ||
7. |
IMMUNOASSAYS :- (Room no. P-19) |
| ||
|
Thyroid Profile – T3, T4, TSH, Anti TPO |
(Prior appointment) Date from Room No. P12 | ||
|
B12, Folic Acid, Ferritin, LH, FSH, Prolactin, Estrogen, Prostate Specific Antigen(PSA), Progesterone, CA-125, Testosterone, CEA, Alpha Fetoprotein, beta-HCG etc. |
OPD collection on Wednesday and Friday Room no. P19 | ||
8. |
HISTOPATHOLOGY:- (ROOM NO. P16) |
| ||
|
Biopsy and excision sample |
In 10% buffered formalin | ||
|
Routine stain (H&E) |
| ||
|
Special Stains: Fite(Leprosy), Iron, Reticulin(Reticulin fibers), SMA(Fungal), PAS(Glycogen), PTAH (Striations), AFB, Congo Red (Amyloid) |
| ||
|
Immunomarkers/Cancer markers |
| ||
|
Duplicate Slide facility to cancer patients for Rtx |
With reference slip of tertiary care hospital | ||
Department of Microbiology
(Facilities available) – No waiting period
1. Aerobic Bacterial Culture And Antibiotic Sensitivity Tests
Ø Blood
Ø Urine
Ø Cerebrospinal fluid
Ø Respiratory secretions
Ø Sputum/ throat swab
Ø Other sterile body fluids ( Pleural/ Synovial/ Ascitic/ Vitreous etc)
Ø Catheters / Endotracheal Tubes
Ø High vaginal swabs
Ø Eye/ ear/ Nasal/ Oral swabs
2. Serology
Ø Widal
Ø VDRL (RPR)
Ø Hepatitis B/ Hepatitis C
Ø HIV in ICTC room near Blood bank
Ø Dengue – NS1, Ig M Mac ELISA
Ø Chikungunya – Ig M
Ø Japanese Encephalitis - Ig M
Ø TORCH
3. Microscopy
Ø Direct Grams stain
Ø ZN stain for AFB (M. tuberculosis & M laprae) except sputum
Ø KOH mount for Fungus
Ø Hanging Drop
Ø Modified ZN stain for Cryptosporidium Spp.
Ø India Ink for Cryptococcus
4. Hospital Infection surveillance
Ø Air sampling/ Swabs
Ø Tube Test for disinfectants sterility tests
CDC link : CDC/NHSN SURVILLANCE DEFINITION FOR SPECIFIC TYPES OF INFECTIONS. www.cdc.govnhsn/pdf/pscmanual/17pscnosinfdef_current.pdf
APPENDIX 1
All Media containing vials , bottles, plates may be collected from microbiology daily during routine sample collection hours i.e. 9.00 AM – 11.45 AM and stored at 4-8o C (for max of 3 days after issue) |
Serum samples for serology |
Collected by venipuncture in plain / clot activator vials with acrylic serum separator gel |
Keep refrigerated at 4-8o C till dispatch to Microbiology lab |
Sterile body fluids (e.g. CSF, pericardial, Pleural, Ascitic, Synovial, vitreous, amniotic etc) |
Collected by site specific puncture. Then inject 2-3 mL in well labelled blood culture bottle+ sterile culture bottle CSF 0.5 mL in BD bottles |
Keep incubated at 35o C till dispatch to Micro Lab |
Blood culture. All blood cultures are collected in media containing bottles. |
Collected by venipuncture in blood culture bottles from atleast 2 different IV sites in two different well labelled bottles. If from central line, then another sample must be taken from another site in a separate blood culture bottles. |
Keep incubated at 35o C till dispatch to Microbiology Lab |
Pus from deep seated abscess |
Collected by needle and syringe in an empty sterile urine culture vials and 0.5- 2 ml pus in blood culture bottle |
Keep incubated at 35o C till dispatch to Microbiology Lab |
Superficial pus from wounds |
Collected by touching with swabs moistened with sterile saline and insert in media (Amies & Stuarts) containing test tubes |
Keep incubated at 35o C till dispatch to Microbiology Lab |
Urine |
Collect either clean catch mid stream urine sample |
Keep refrigerated at 4-8o C till dispatch to Microbiology lab |
Urine |
Or fresh drops from clamped catheter or suprapubic sample aseptically collected in a sterile culture vials. |
Rapid transport to Micro Lab |